MTSviewer

Welcome to MTSviewer, a database of mitochondrial proteins integrating:

- MTS predictions
- MPP cleavage sites
- Genetic variants
- Pathogenicity predictions
- N-terminomics data
- Structural visualization using AlphaFold models

Using this platform, we have generated a list of disease-linked variants in protein MTS's and their predicted consequences as a resource for their functional characterization. Overall, MTSviewer is a platform that can be used to interrogate MTS mutations and their potential effects on import and proteolysis across the mitochondrial proteome.

Click on the MTSviewer tab and start interrogating your favorite protein(s) listed in the dropdown menu!

Frequently Asked Questions

Structures are taken from AlphaFold, using the human proteome from 21 July 2021. We will update our viewer with the latest AlphaFold updates as they come out.

In this version of MTSviewer, we are only taking proteins from the MitoCarta 3.0 for human proteins, and 901 high-confidence yeast mitochondrial proteins via Morgernstern et al. 2017. In later versions, we hope to expand this viewer to the entire human proteome, as cytosolic proteins can also contain iMTS's worth studying.

Variants are parsed by Uniprot ID through the dbNSFP v4.2a, and then filtered and matched to the VEP canonical transcript. All genomic coordinates correspond to GRCH38/hg38.

v3.1, derived from dbNSFP v4.2a

Currently they are derived from TopFIND 4.1.

All cleavage sites on the iMTS plot are given by a single a.a. number, which corresponds to the amino acid that is C-terminal to the cleaved peptide bond. For example, '5' implies that the cleavage site is between a.a. 5 and 6 (ie. 12345|6789). For TopFIND N-terminomics sites (TopFINDnt), we have converted their a.a. numbering to match this format on the iMTS plot. The equivalent cleavage sites in the plaintext TopFIND tables are reported as originally indicated by their peptide idenficiation numbering (ie. iMTS plot cleavage site + 1).

The current version of MTSviewer features the inherent limitation that N-terminal MTS's within AlphaFold predictions are typically low confidence and are depicted as unstructured. In the future, structural determination of human MTS's in complexes with TOM/TIM and/or MPP will enable us to model N-MTS's more accurately, and could be integrated as a scoring metric or docking module into later versions of MTSviewer

Please direct all inquiries to our GitHub or directly to our e-mail address.

Welcome to MTSviewer, a database of mitochondrial proteins integrating:

iMLP curves

Sequence

Mutation List

Download

Cleavage Site Predictions

MitoFates

TargetP

TPPred3

TopFind

DeepMito

Frequently Asked Questions

To report bugs, comments, or to submit custom proteins to be added to MTSviewer, please e-mail:

mtsviewer.docs@gmail.com

If you are requesting specific proteins to be added to MTSViewer, please attach a FASTA file of interest.

When using MTSviewer please cite:

MTSviewer: a database to visualize mitochondrial targeting sequences, cleavage sites, and mutations on protein structures
Andrew N. Bayne, Jing Dong, Saeid Amiri, Sali M.K. Farhan, Jean-Francois Trempe (2023)
PLOS ONE 18(4): e0284541.
DOI: https://doi.org/10.1371/journal.pone.0284541